Anticorps Polyclonal de lapin anti-Ajuba

Ajuba Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 28686-1-AP

Synonymes

Ajuba, JUB, Protein ajuba

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Galerie de données de validation

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  WB analysis using 28686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28686-1-AP (Ajuba antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28686-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu cutané de souris, tissu cutané de rat, tissu de cancer de la thyroïde humain, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28686-1-AP cible Ajuba dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ajuba Protéine recombinante Ag29725
• Nom complet: jub, ajuba homolog (Xenopus laevis)
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC007580
• Symbole du gène: Ajuba
• Identification du gène (NCBI): 84962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.

Publications

Species	Application	Title
human	WB	Cell Rep; ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression; Authors - Yu Zhang

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