Anticorps Monoclonal anti-Alpha E-Catenin

Alpha E-Catenin Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2B7B8

N° de cat : CL594-66221

Synonymes

Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, FLJ36832, FLJ52416

---

Galerie de données de validation

• 

  FC experiment of MCF-7 using CL594-66221

  1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha E-Catenin (CL594-66221, Clone:2B7B8) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en FC (Intra): cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66221 cible Alpha E-Catenin dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Alpha E-Catenin Protéine recombinante Ag23603
• Nom complet: catenin (cadherin-associated protein), alpha 1, 102kDa
• Masse moléculaire calculée: 906 aa, 100 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC031262
• Symbole du gène: Alpha E-Catenin
• Identification du gène (NCBI): 1495
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

{{ptg:RelatedPrimaryAntibodies}}