Anticorps Recombinant de lapin anti-Alpha Tubulin

• Phare
• Validé par KD/KO

Alpha Tubulin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

1G7

Avis (1)

N° de cat : 80762-1-RR

Synonymes

TUBA1B, Tubulin alpha 1B chain, 1G7, Alpha-tubulin ubiquitous, EC:3.6.5.-

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Galerie de données de validation

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  WB analysis using 80762-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 80762-1-RR

  WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells.

• 

  IHC staining of mouse brain using 80762-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 80762-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 80762-1-RR

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 80762-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 80762-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of C2C12 using 80762-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 80762-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 80762-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80762-1-RR against Human Alpha Tubulin were performed. The affinity constant is 0.708 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cerveau de rat, cerveau de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF-Fro: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules C2C12, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

80762-1-RR cible Alpha Tubulin dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 10376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	WB	Dose Response; The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.; Authors - Danjie Liu
mouse	WB	Mol Med Rep; Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium; Authors - Cheng Chen
human	WB	Mol Med; Liraglutide attenuates type 2 diabetes mellitus-associated non-alcoholic fatty liver disease by activating AMPK/ACC signaling and inhibiting ferroptosis; Authors - Tingli Guo
human,mouse	WB	Cells; Melatonin Ameliorates Post-Stroke Cognitive Impairment in Mice by Inhibiting Excessive Mitophagy; Authors - Yan Shi
mouse	WB	Exp Neurol; SARS-CoV-2 spike S1 protein induces microglial NLRP3-dependent neuroinflammation and cognitive impairment in mice; Authors - Qiuhong Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (07-24-2025)

We observed consistent and robust signal intensity across multiple replicates, indicating good reproducibility.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H9C2 cells， liver

Alpha Tubulin Antibody Western Blot validation (1:5000 dilution) in H9C2 cells， liver (Cat no:80762-1-RR)

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