Anticorps Recombinant de lapin anti-Alpha Tubulin

• Phare
• Validé par KD/KO

Alpha Tubulin Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1G7

N° de cat : CL594-80762

Synonymes

TUBA1B, Tubulin alpha 1B chain, 1G7, Alpha-tubulin ubiquitous, EC:3.6.5.-

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Galerie de données de validation

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (orange), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, green). Nuclei shown in red. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, red). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of HepG2 using CL594-80762

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Alpha Tubulin antibody (CL594-80762, Clone: 1G7 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-80762

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Alpha Tubulin Recombinant antibody (CL594-80762, Clone:1G7)(red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, human adipose-derived mesenchymal stem cells
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-80762 cible Alpha Tubulin dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag18034
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 10376
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

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