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Anticorps Monoclonal anti-Beta-2-Microglobulin

Beta-2-Microglobulin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1C3B7

N° de cat : 66207-1-Ig

Synonymes

B2M, Beta-2-microglobulin, HDCMA22P

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis using 66207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    WB analysis of Raji using 66207-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human prostate cancer using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IHC staining of human renal cell carcinoma using 66207-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Beta-2-Microglobulin Monoclonal antibody (66207-1-Ig)

    IF Staining of human prostate cancer using 66207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Applications testées

Résultats positifs en WBcellules A431, cellules LNCaP, cellules Raji
Résultats positifs en IHCtissu de cancer de la prostate humain, tissu de carcinome à cellules rénales humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFtissu de cancer de la prostate humain,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochimie (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66207-1-Ig cible Beta-2-Microglobulin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet beta-2-microglobulin
Masse moléculaire calculée 14 kDa
Poids moléculaire observé 13 kDa
Numéro d’acquisition GenBankBC032589
Symbole du gène B2M
Identification du gène (NCBI) 567
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocole

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
IF protocol for Beta-2-Microglobulin antibody 66207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Santhosh (Verified Customer) (04-15-2019)

Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
Beta-2-Microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)