Anticorps Polyclonal de lapin anti-B4GALT6

• Phare
• Validé par KD/KO

B4GALT6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20148-1-AP

Synonymes

EC:2.4.1.274, EC:2.4.1.-, Beta4Gal-T6, Beta-1,4-GalTase 6, Beta-1,4-galactosyltransferase 6

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Galerie de données de validation

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  WB analysis of HEK-293 using 20148-1-AP

  WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells.

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  WB analysis of mouse heart using 20148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20148-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20148-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20148-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 20148-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 20148-1-AP

  Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

20148-1-AP cible B4GALT6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B4GALT6 Protéine recombinante Ag13686
• Nom complet: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• Masse moléculaire calculée: 382 aa, 45 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC074884
• Symbole du gène: B4GALT6
• Identification du gène (NCBI): 9331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).

Publications

Species	Application	Title
mouse	IF	Nat Med; Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation.; Authors - Lior Mayo
human	IHC	Biochim Biophys Acta Mol Cell Biol Lipids; Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors.; Authors - Jiřina Procházková
human,mouse	IF	Cell Metab; Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex; Authors - Chenxi Zhao

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