Anticorps Monoclonal anti-BACH1

• Phare
• Validé par KD/KO

BACH1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2D12A4

N° de cat : 66762-1-Ig

Synonymes

2D12A4, BACH 1, BACH-1

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Galerie de données de validation

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  WB analysis using 66762-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 66762-1-Ig

  WB result of BACH1 antibody (66762-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells.

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  WB analysis of HSC-T6 using 66762-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules Raji
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

66762-1-Ig cible BACH1 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BACH1 Protéine recombinante Ag6011
• Nom complet: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC063307
• Symbole du gène: BACH1
• Identification du gène (NCBI): 571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Publications

Species	Application	Title
mouse,human	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin ; KD Validated
human	WB	Life Sci Alliance; A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95; Authors - Kristen M Rehl
mouse	IF	Cell Mol Biol Lett; MAFF alleviates hepatic ischemia-reperfusion injury by regulating the CLCF1/STAT3 signaling pathway; Authors - Dengliang Lei
mouse	WB,IF,ChIP	Exp Neurol; Gastrodin alleviates Aβ25-35-induced glycolytic dysfunction via activating PI3K/AKT/BACH1 signaling in Alzheimer's disease models; Authors - Ting-Ting Liu
human	WB	Cancer Sci; BACH1 promotes clear cell renal cell carcinoma progression by upregulating oxidative stress-related tumorigenicity; Authors - Kenshiro Takemoto ; KD Validated

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