Anticorps Polyclonal de lapin anti-BAK

• Phare
• Validé par KD/KO

BAK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(24)

N° de cat : 29552-1-AP

Synonymes

BAK1, Bcl-2 homologous antagonist/killer, Bcl2-L-7, Bcl-2-like protein 7, CDN1

---

Galerie de données de validation

• 

  WB analysis using 29552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29552-1-AP (BAK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29552-1-AP (BAK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 29552-1-AP

  WB result of BAK antibody (29552-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAK transfected A431 cells.

• 

  IP experiment of A431 using 29552-1-AP

  IP result of anti-BAK (IP:29552-1-AP, 4ug; Detection:29552-1-AP 1:3000) with A431 cells lysate 1040 ug.

• 

  IHC staining of human colon cancer using 29552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29552-1-AP (BAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 29552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29552-1-AP (BAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 29552-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAK antibody (29552-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules 4T1, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MOLT-4, cellules Neuro-2a, cellules ROS1728
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 4 publications below

Informations sur le produit

29552-1-AP cible BAK dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAK Protéine recombinante Ag31042
• Nom complet: BCL2-antagonist/killer 1
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: NM_001188
• Symbole du gène: BAK1
• Identification du gène (NCBI): 578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Publications

Species	Application	Title
human	WB	Apoptosis; Investigation of GPR143 as a promising novel marker for the progression of skin cutaneous melanoma through bioinformatic analyses and cell experiments; Authors - Ruimin Bai
human	WB	Life Sci; A novel antimycin analogue antimycin A2c, derived from marine Streptomyces sp., suppresses HeLa cells via disrupting mitochondrial function and depleting HPV oncoproteins E6/E7; Authors - Weiyi Zhang
human	WB	Mar Drugs; Astaxanthin Inhibits H2O2-Induced Excessive Mitophagy and Apoptosis in SH-SY5Y Cells by Regulation of Akt/mTOR Activation; Authors - Tingting Yan
chicken	WB	Poult Sci; HSP60 inhibits DF-1 apoptosis through its mitochondrial signal peptide; Authors - Shengliang Cao
mouse	WB	Cell Metab; Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining; Authors - Ya Liu
human	WB	Cell Biochem Biophys; MiR-218 Exhibits Anti-Leukemia Effects by Targeting CTNND2 in Primary Acute Erythroid Leukemia HEL Cells; Authors - Ming-Qiang Chu

{{ptg:RelatedPrimaryAntibodies}}