Anticorps Polyclonal de lapin anti-BAK

BAK Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 30017-1-AP

Synonymes

BAK1, CDN1, Bcl-2-like protein 7, Bcl2-L-7, Bcl-2 homologous antagonist/killer

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Galerie de données de validation

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  WB analysis using 30017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 30017-1-AP

  IP result of anti-BAK (IP:30017-1-AP, 4ug; Detection:30017-1-AP 1:6000) with HEK-293 cells lysate 1640 ug.

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  IF Staining of HeLa using 30017-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAK antibody (30017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Neuro-2a, cellules PC-12, cellules THP-1
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

30017-1-AP cible BAK dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAK Protéine recombinante Ag27958
• Nom complet: BCL2-antagonist/killer 1
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: BC004431
• Symbole du gène: BAK1
• Identification du gène (NCBI): 578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Publications

Species	Application	Title
duck	WB	J Virol; Duck Tembusu virus NS3 protein induces apoptosis by activating the PERK/PKR pathway and mitochondrial pathway; Authors - Yuhong Pan

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