Anticorps Polyclonal de lapin anti-BBS4

• Phare
• Validé par KD/KO

BBS4 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 12766-1-AP

Synonymes

Bardet Biedl syndrome 4, BBS4

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Galerie de données de validation

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  WB analysis using 12766-1-AP

  HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12766-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12766-1-AP (BBS4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12766-1-AP (BBS4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 12766-1-AP

  IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 10 publications below

Informations sur le produit

12766-1-AP cible BBS4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS4 Protéine recombinante Ag3430
• Nom complet: Bardet-Biedl syndrome 4
• Masse moléculaire calculée: 519 aa, 58 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC027624
• Symbole du gène: BBS4
• Identification du gène (NCBI): 585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

Publications

Species	Application	Title
human	IF	Nat Commun; Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.; Authors - Dragomir B Krastev
mouse	WB,IF	PLoS Biol; Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.; Authors - Naila Haq ; KO Validated
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
mouse	IF	Autophagy; The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.; Authors - Andreas Struchtrup
human	WB	EMBO Rep; Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.; Authors - Melis D Arslanhan
mouse	WB	Elife; Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.; Authors - Yan Xiong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T