Anticorps Recombinant de lapin anti-BCAN

BCAN Recombinant Antibody for FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230126D6

N° de cat : 82956-1-RR

Synonymes

Brevican, 230126D6, BEHAB, Brain-enriched hyaluronan-binding protein, Brevican core protein

---

Galerie de données de validation

• 

  FC experiment of SH-SY5Y using 82956-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human BCAN (82956-1-RR, Clone:230126D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82956-1-RR cible BCAN dans les applications de FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BCAN Protéine recombinante Ag13596
• Nom complet: brevican
• Masse moléculaire calculée: 99 kDa
• Numéro d’acquisition GenBank: BC010571
• Symbole du gène: BCAN
• Identification du gène (NCBI): 63827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

{{ptg:RelatedPrimaryAntibodies}}