Anticorps Polyclonal de lapin anti-BAP31

• Phare
• Validé par KD/KO

BAP31 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (1)

N° de cat : 11200-1-AP

Synonymes

BCAP31, 6C6 AG, 6C6-AG tumor-associated antigen, B-cell receptor-associated protein 31, BCR-associated protein 31

---

Galerie de données de validation

• 

  WB analysis using 11200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 11200-1-AP

  IP result of anti-BAP31 (IP:11200-1-AP, 3ug; Detection:11200-1-AP 1:1000) with A431 cells lysate 3300ug.

• 

  IHC staining of human breast cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (11200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), phalloidine stains F-actin (red).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules THP-1, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

11200-1-AP cible BAP31 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAP31 Protéine recombinante Ag1668
• Nom complet: B-cell receptor-associated protein 31
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC014323
• Symbole du gène: BAP31
• Identification du gène (NCBI): 10134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Publications

Species	Application	Title
	WB	Nat Biotechnol; Efficient proximity labeling in living cells and organisms with TurboID.; Authors - Tess C Branon
human	WB	Nat Methods; Directed evolution of APEX2 for electron microscopy and proximity labeling.; Authors - Stephanie S Lam
human	WB	Nat Commun; HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.; Authors - Ulrike Zinnall
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu
mouse	WB	Mol Syst Biol; Subcellular mRNA kinetic modeling reveals nuclear retention as rate-limiting; Authors - David Steinbrecht
human	WB,IP	EMBO J; Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.; Authors - Iwasawa Ryota R ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Boyan (Verified Customer) (11-02-2020)

This antibody labeled the ER area clearly

• Applications: Immunofluorescence

{{ptg:RelatedPrimaryAntibodies}}