Anticorps Polyclonal de lapin anti-BCAT1

• Phare
• Validé par KD/KO

BCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(41)

Avis (2)

N° de cat : 13640-1-AP

Synonymes

PNAS 121, MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic

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Galerie de données de validation

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  WB analysis using 13640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13640-1-AP

  WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells.

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  IHC staining of mouse brain using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13640-1-AP (BCAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13640-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCAT1 antibody (13640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules Neuro-2a
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 34 publications below
• IHC: See 13 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

13640-1-AP cible BCAT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCAT1 Protéine recombinante Ag4574
• Nom complet: branched chain aminotransferase 1, cytosolic
• Masse moléculaire calculée: 320 aa, 36 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC033864
• Symbole du gène: BCAT1
• Identification du gène (NCBI): 586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian ; KO Validated
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human	WB, IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang ; KO Validated
human	WB,IHC	Oncotarget; BCAT1 expression associates with ovarian cancer progression: possible implications in altered disease metabolism.; Authors - Zhi-Qiang Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kis (Verified Customer) (12-08-2022)

This antibody was used for (1:1000) WB and (1:500) for IHC. We got satisfactory results with this ab.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

BCAT1 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Human Liver cells (Cat no:13640-1-AP)

FH

Iru (Verified Customer) (06-18-2020)

It works fine with FTE cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

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