Anticorps Polyclonal de lapin anti-BCAT2

• Phare
• Validé par KD/KO

BCAT2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (2)

N° de cat : 16417-1-AP

Synonymes

BCAT2, ECA40, Placental protein 18, PP18

---

Galerie de données de validation

• 

  WB analysis using 16417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16417-1-AP

  WB result of BCAT2 antibody (16417-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT2 transfected MCF-7 cells.

• 

  WB analysis of L02 using 16417-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 16417-1-AP (BCAT2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 16417-1-AP (BCAT2 Antibody) at dilution of 1:50.

• 

  IF Staining of C2C12 using 16417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16417-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human BCAT2 (16417-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, cellules MCF-7, cellules Raji
• Résultats positifs en IHC: tissu cutané humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules C2C12,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

16417-1-AP cible BCAT2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCAT2 Protéine recombinante Ag9573
• Nom complet: branched chain aminotransferase 2, mitochondrial
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC001900
• Symbole du gène: BCAT2
• Identification du gène (NCBI): 587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

Publications

Species	Application	Title
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human,mouse	WB	Nat Commun; Glucose promotes cell growth by suppressing branched-chain amino acid degradation.; Authors - Dan Shao
human	WB, IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kamal (Verified Customer) (02-15-2024)

Antibody works well when diluted in 1X TBST. Bands appeared at 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

chao (Verified Customer) (01-30-2020)

Strong, clear band for heart lysates.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart