Anticorps Polyclonal de lapin anti-BCCIP

• Phare
• Validé par KD/KO

BCCIP Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16043-1-AP

Synonymes

BCCIP, Protein TOK 1, TOK 1, TOK1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 16043-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16043-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16043-1-AP

  IP Result of anti-BCCIP (IP:16043-1-AP, 4ug; Detection:16043-1-AP 1:800) with HeLa cells lysate 3200ug.

• 

  IF Staining of HeLa using 16043-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16043-1-AP (BCCIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

16043-1-AP cible BCCIP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCCIP Protéine recombinante Ag8934
• Nom complet: BRCA2 and CDKN1A interacting protein
• Masse moléculaire calculée: 314 aa, 36 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC009771
• Symbole du gène: BCCIP
• Identification du gène (NCBI): 56647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; The Males Absent on the First (MOF) Mediated Acetylation Alters the Protein Stability and Transcriptional Activity of YY1 in HCT116 Cells; Authors - Tingting Wu
human	WB	Int J Mol Sci; Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells; Authors - Junaid Ali Shah
human	WB,IF	FEBS J; BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.; Authors - Yi Sui ; KD Validated
human	WB,IF	Protein Cell; Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.; Authors - Jiaming Su
human	WB	Biochem Biophys Res Commun; BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.; Authors - Fang Wang
human	WB,IHC	Int J Oncol; Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues.; Authors - Liu Xiaoxia X