Anticorps Polyclonal de lapin anti-BCL11B

BCL11B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 55414-1-AP

Synonymes

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

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Galerie de données de validation

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  WB analysis of Jurkat using 55414-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 55414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 55414-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55414-1-AP

  IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug.

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  IHC staining of mouse brain using 55414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55414-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, tissu de thymus de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

55414-1-AP cible BCL11B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: B-cell CLL/lymphoma 11B (zinc finger protein)
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: NM_022898
• Symbole du gène: BCL11B
• Identification du gène (NCBI): 64919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

Publications

Species	Application	Title
Mouse		Cell Rep; XLF/Cernunnos loss impairs mouse brain development by altering symmetric proliferative divisions of neural progenitors; Authors - Amandine Bery
human	WB	Arch Virol; Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.; Authors - Juan Wang
mouse	WB,IF	EMBO J; BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells; Authors - Yehua Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

Good for ICC on subcerebral neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs

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