Anticorps Polyclonal de lapin anti-human BCL2

• Phare
• Validé par KD/KO

human BCL2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (8)

Applications

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

2207

Avis (6)

N° de cat : 12789-1-AP

Synonymes

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

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Galerie de données de validation

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  WB analysis of A431 using 12789-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat cells using 12789-1-AP

  WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.

• 

  WB analysis using 12789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (human BCL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12789-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 12789-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12789-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 12789-1-AP

  IP result of anti-BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.

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  IP experiment of HepG2 using 12789-1-AP

  IP Result of anti-BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:500) with HepG2 cells lysate 4500ug.

• 

  IP experiment of MCF-7 using 12789-1-AP

  IP Result of anti-BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

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  IHC staining of human tonsillitis using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 12789-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human BCL2 (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human peripheral blood lymphocytes using 12789-1-AP

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.2 ug Anti-Human BCL2 (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HepG2, cellules HL-60, cellules Jurkat, cellules MCF-7, cellules U-251, cellules U2OS, cellules U-937
• Résultats positifs en IP: cellules HL-60, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat, lymphocytes de sang périphérique humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:300
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2134 publications below
• IHC: See 103 publications below
• IF: See 36 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 6 publications below
• ChIP: See 1 publications below

Informations sur le produit

12789-1-AP cible human BCL2 dans les applications de WB, IP, IHC, IF, FC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain, Lapin, poisson-zèbre, porc, poulet, Hamster, duck, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: human BCL2 Protéine recombinante Ag3508
• Nom complet: B-cell CLL/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC027258
• Symbole du gène: BCL2
• Identification du gène (NCBI): 596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
	WB,CoIP	Cell Metab; Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise; Authors - Kenta Kuramoto
pig	WB	Cell Stem Cell; Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation; Authors - Jiaowei Wang
human	WB	ACS Nano; Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer.; Authors - Danielle M Valcourt
	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-21-2023)

The antibody works well in recognising Bcl-2 in western blots. Great product!

• Applications: Western Blot
• Cell Tissue Type: HUVEC

FH

Jia (Verified Customer) (03-01-2022)

The antibody works well for Bcl2, we recommend it.

• Applications: Western Blot

FH

Giyeong (Verified Customer) (02-18-2022)

Works well with sharp distinct vand

• Applications: Western Blot

FH

Morgan (Verified Customer) (10-26-2021)

This product worked really well. I attached photos to show how clean the blot came out when used on ovarian cancer cells (OV90s). I used 1:500 for the first use, but I could definitely use less antibody next time.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 (Ovarian cancer cell line)

human BCL2 Antibody Western Blot validation (1:500 dilution) in OV90 (Ovarian cancer cell line) (Cat no:12789-1-AP)

FH

Tom (Verified Customer) (10-09-2020)

This antibody recognizes Bcl-2 at the correct molecular weight. Highly recommended!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,05µl/10^6 cells
• Cell Tissue Type: B cells

human BCL2 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:12789-1-AP)

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