Anticorps Monoclonal anti-BCL2

• Phare
• Validé par KD/KO

BCL2 Monoclonal Antibody for IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc et plus (4)

Applications

IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(351)

CloneNo.

4H8C6

N° de cat : 60178-1-Ig

Synonymes

BCL-2, 4H8C6

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Galerie de données de validation

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  IP experiment of U-937 using 60178-1-Ig

  IP result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug.

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  IHC staining of human appendicitis using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Jurkat using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human tonsillitis using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human lymphoma using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human appendicitis using 60178-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60178-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 60178-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IP: cellules U-937
• Résultats positifs en IHC: tissu d'appendicite humain, cellules Jurkat, tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'appendicite humain, tissu d'amygdalite humain
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:6400-1:25600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 238 publications below
• IHC: See 20 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

60178-1-Ig cible BCL2 dans les applications de IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: bovin, Humain, Lapin, porc, singe, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCL2 Protéine recombinante Ag3508
• Nom complet: B-cell CLL/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC027258
• Symbole du gène: BCL2
• Identification du gène (NCBI): 596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
	IHC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IP	EMBO J; VHL suppresses autophagy and tumor growth through PHD1-dependent Beclin1 hydroxylation; Authors - Zheng Wang
	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
	WB	Redox Biol; Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats.; Authors - Haiyan Fan

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