Anticorps Polyclonal de lapin anti-BCL2L13

• Phare
• Validé par KD/KO

BCL2L13 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 16612-1-AP

Synonymes

BCL2L13, MIL1, Protein Mil1

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Galerie de données de validation

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  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16612-1-AP

  IP Result of anti-BCL2L13 (IP:16612-1-AP, 3ug; Detection:16612-1-AP 1:100) with mouse heart tissue lysate 8000ug.

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

16612-1-AP cible BCL2L13 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCL2L13 Protéine recombinante Ag9841
• Nom complet: BCL2-like 13 (apoptosis facilitator)
• Masse moléculaire calculée: 485 aa, 53 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC003032
• Symbole du gène: BCL2L13
• Identification du gène (NCBI): 23786
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).

Publications

Species	Application	Title
mouse	IHC	Cancer Commun (Lond); Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites; Authors - Ji Eon Kim
human	WB	Nat Commun; Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.; Authors - Tomokazu Murakawa
human,mouse	WB	Autophagy; BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.; Authors - Xiaoli Wu
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB,IHC,IF	Proc Natl Acad Sci U S A; Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.; Authors - Samuel A Jensen ; KD Validated
mouse,rat	IF	Glia; Neuroglial transmitophagy and Parkinson's disease.; Authors - Ingrid Morales

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Melissa (Verified Customer) (07-28-2022)

Antibody worked well in western blot. Lysate used was from Huh7 cells IP-ed for a different target protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Huh7 cells

BCL2L13 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:16612-1-AP)