Anticorps Polyclonal de lapin anti-BCL6

• Phare
• Validé par KD/KO

BCL6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 21187-1-AP

Synonymes

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

---

Galerie de données de validation

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 21187-1-AP

  IP Result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.

• 

  IHC staining of human lymphoma using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  FC experiment of Ramos using 21187-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human BCL6 (21187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en WB: cellules Raji
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

21187-1-AP cible BCL6 dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCL6 Protéine recombinante Ag15519
• Nom complet: B-cell CLL/lymphoma 6
• Masse moléculaire calculée: 706 aa, 79 kDa
• Poids moléculaire observé: 79-95 kDa
• Numéro d’acquisition GenBank: BC150184
• Symbole du gène: BCL6
• Identification du gène (NCBI): 604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Publications

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
human	WB	Respir Res; Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.; Authors - Ziyan Yan
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	WB,IP	Front Cell Dev Biol; Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma; Authors - Mayuko Nishi
human	IHC	Front Cell Dev Biol; The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.; Authors - Zhengyan Chang
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011