Anticorps Polyclonal de lapin anti-BCR

• Phare
• Validé par KD/KO

BCR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 22585-1-AP

Synonymes

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, PHL

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Galerie de données de validation

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  WB analysis of Y79 using 22585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 22585-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 22585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22585-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22585-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22585-1-AP

  IP Result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

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  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22585-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22585-1-AP (BCR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 22585-1-AP

  Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 22585-1-AP

  1X10^6 HepG2 cells were stained with .2ug BCR antibody (22585-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules COS-7, cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

22585-1-AP cible BCR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCR Protéine recombinante Ag18281
• Nom complet: breakpoint cluster region
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC066122
• Symbole du gène: BCR
• Identification du gène (NCBI): 613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Publications

Species	Application	Title
human,mouse	WB,IHC	Neurogastroenterol Motil; Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.; Authors - Yongtao Xiao ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100