Anticorps Monoclonal anti-BCR

BCR Monoclonal Antibody for WB, ELISA, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, ELISA, FC (Intra)

Conjugaison

Non conjugué

CloneNo.

2C2E3

N° de cat : 67260-1-Ig

Synonymes

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

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Galerie de données de validation

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  WB analysis of Jurkat using 67260-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67260-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67260-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 67260-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa
• Résultats positifs en FC (Intra): cellules K-562
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67260-1-Ig cible BCR dans les applications de WB, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCR Protéine recombinante Ag28953
• Nom complet: breakpoint cluster region
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC066122
• Symbole du gène: BCR
• Identification du gène (NCBI): 613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

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