Anticorps Polyclonal de lapin anti-BCRP/ABCG2

• Phare
• Validé par KD/KO

BCRP/ABCG2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(66)

Avis (3)

N° de cat : 27286-1-AP

Synonymes

ABCG2, BCRP, BCRP,ABCG2, ABC15, ABCP

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Galerie de données de validation

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  WB analysis of MCF-7 using 27286-1-AP

  WB result of BCRP,ABCG2 antibody (27286-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCRP,ABCG2 transfected MCF-7 cells.

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  WB analysis using 27286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules MCF-7, cellules SGC-7901, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 59 publications below
• IHC: See 15 publications below
• IF: See 5 publications below

Informations sur le produit

27286-1-AP cible BCRP/ABCG2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCRP/ABCG2 Protéine recombinante Ag25911
• Nom complet: ATP-binding cassette, sub-family G (WHITE), member 2
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC021281
• Symbole du gène: ABCG2
• Identification du gène (NCBI): 9429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Publications

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
mouse,human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
mouse,human	WB,IHC	PLoS Biol; A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance; Authors - Jiaojiao Hao
human	WB	Cancer Lett; Lovastatin/SN38 co-loaded liposomes amplified ICB therapeutic effect via remodeling the immunologically-cold colon tumor and synergized stimulation of cGAS-STING pathway; Authors - Yi Yang
mouse,human	WB,IHC	Mol Ther; CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma.; Authors - Yuanyuan Ma
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (07-24-2024)

Incubation with primary antibody ON at 4 C. Incubation with secondary antibody (rabbit) 2h at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell lines

FH

Caitlin (Verified Customer) (07-12-2024)

The antibody worked well in that there was minimal background staining. However, the protein shows as multiple bands. Although, this is possibly due to a protein preparation issue rather than an antibody issue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A2780

FH

Udesh (Verified Customer) (09-12-2022)

Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS Osteosarcoma cells

BCRP/ABCG2 Antibody Western Blot validation (1:3000 dilution) in U2OS Osteosarcoma cells (Cat no:27286-1-AP)

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