Anticorps Polyclonal de lapin anti-Biglycan

• Phare
• Validé par KD/KO

Biglycan Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 16409-1-AP

Synonymes

A6NLG9, BGN, biglycan, Bone/cartilage proteoglycan I, DSPG1, PG S1, PGI, SLRR1A

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Galerie de données de validation

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  WB analysis using 16409-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16409-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cartilage using 16409-1-AP

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16409-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16409-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16409-1-AP

  Immunofluorescent analysis of HepG2 cells using 16409-1-AP (Biglycan antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HepG2, tissu cartilagineux de porc, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 7 publications below

Informations sur le produit

16409-1-AP cible Biglycan dans les applications de WB, IHC, IF, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Biglycan Protéine recombinante Ag0683
• Nom complet: biglycan
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC004244
• Symbole du gène: BGN
• Identification du gène (NCBI): 633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Publications

Species	Application	Title
human	WB,IHC	Oncoimmunology; Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.; Authors - Shaoquan Zheng
human	WB,IF	Cancers (Basel); Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.; Authors - Kanakaraju Manupati ; KD Validated
human	WB,IHC	Oncoimmunology; Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.; Authors - Karthikeyan Subbarayan
rat	IF	Cell Prolif; Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.; Authors - Yunjiao Wang
human	IHC	Cancer Med; Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma; Authors - Wanyi Xiao
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chronogenic Kit Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Placenta healthy

Biglycan Antibody Immunohistochemistry validation (1:500 dilution) in Placenta healthy (Cat no:16409-1-AP)

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