Anticorps Polyclonal de lapin anti-Biglycan

• Phare
• Validé par KD/KO

Biglycan Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(28)

Avis (2)

N° de cat : 16409-1-AP

Synonymes

BGN, PRG1, PG-S1, PGI, PG S1

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Galerie de données de validation

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  WB analysis using 16409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig cartilage using 16409-1-AP

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16409-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16409-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16409-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human normal colon using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16409-1-AP (Biglycan antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse lung using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver cancer using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16409-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Biglycan antibody (16409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HepG2, tissu cartilagineux de porc, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu hépatique humain, tissu pulmonaire de souris, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:200-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 9 publications below
• IF: See 10 publications below

Informations sur le produit

16409-1-AP cible Biglycan dans les applications de WB, IHC, IF/ICC, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Biglycan Protéine recombinante Ag0683
• Nom complet: biglycan
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC004244
• Symbole du gène: Biglycan
• Identification du gène (NCBI): 633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Publications

Species	Application	Title
human	WB,IHC	Oncoimmunology; Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.; Authors - Shaoquan Zheng
human	WB,IF	Cancers (Basel); Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.; Authors - Kanakaraju Manupati ; KD Validated
mouse	IHC	J Biol Eng; Proteomic analysis of decellularized mice liver and kidney extracellular matrices; Authors - Anna-Maria Diedrich
human	WB,IHC	Oncoimmunology; Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.; Authors - Karthikeyan Subbarayan
rat	IF	Cell Prolif; Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.; Authors - Yunjiao Wang
human	IHC	Cancer Med; Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma; Authors - Wanyi Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lin (Verified Customer) (11-18-2024)

Cells were fixed in 4% PFA, permeabilized with .1% Triton, and blocked for 1 hour. 1o was incubated overnight in 1% BSA followed by 2o incubation for 1hr and imaging. More concentrated dilutions than 1:500 (eg 1:200) produced nonspecific aggregates outside of cells that were not seen in 2o only control.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MC3T3 cells (mouse pre-osteoblasts), not treated with Ascorbic acid

FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chronogenic Kit Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Placenta healthy

Biglycan Antibody Immunohistochemistry validation (1:500 dilution) in Placenta healthy (Cat no:16409-1-AP)

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