Anticorps Monoclonal anti-TGFBI / BIGH3

• Phare
• Validé par KD/KO

TGFBI / BIGH3 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

3E11D11

N° de cat : 60007-1-Ig

Synonymes

TGFBI, 3E11D11, Beta ig h3, Beta ig-h3, BIGH3

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Galerie de données de validation

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  WB analysis of human kidney using 60007-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60007-1-Ig

  IP result of anti-TGFBI / BIGH3 (IP:60007-1-Ig, 4ug; Detection:60007-1-Ig 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human colon using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 60007-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 60007-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  Affinity and Kinetic Characterization of 60007-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60007-1-Ig against Human TGFBI / BIGH3 were performed. The affinity constant is 1.64 nM.

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Applications testées

• Résultats positifs en WB: tissu rénal humain,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

60007-1-Ig cible TGFBI / BIGH3 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGFBI / BIGH3 Protéine recombinante Ag0241
• Nom complet: transforming growth factor, beta-induced, 68kDa
• Masse moléculaire calculée: 683 aa, 75 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC000097
• Symbole du gène: TGFBI
• Identification du gène (NCBI): 7045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Publications

Species	Application	Title
mouse,human	WB,IHC	Cancer Res; TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.; Authors - Laura S M Lecker
human	IF	Cell Rep; Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury; Authors - Yiheng Mao
human	WB,IHC	Int J Cancer; Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.; Authors - Ween Miranda P MP
human	WB	Cell Mol Life Sci; TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.; Authors - Irigoyen M M
human	WB	Front Oncol; Identification of Hub Genes Associated With Sensitivity of 5-Fluorouracil Based Chemotherapy for Colorectal Cancer by Integrated Bioinformatics Analysis.; Authors - Ya Wang
human	WB,IHC	Neoplasia; BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.; Authors - Tianhong Pan ; KD Validated

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