Anticorps Monoclonal anti-BLNK

BLNK Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A1A1

N° de cat : CL594-66953

Synonymes

SLP65, Cytoplasmic adapter protein, B-cell linker protein, B-cell adapter containing a Src homology 2 domain protein, B-cell adapter containing a SH2 domain protein

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Galerie de données de validation

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  IF Staining of NIH/3T3 using CL594-66953

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 BLNK antibody (CL594-66953, Clone: 1A1A1 ) at dilution of 1:200.

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  FC experiment of Daudi using CL594-66953

  1X10^6 Daudi cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BLNK (CL594-66953, Clone:1A1A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules Daudi

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66953 cible BLNK dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BLNK Protéine recombinante Ag28673
• Nom complet: B-cell linker
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC018906
• Symbole du gène: BLNK
• Identification du gène (NCBI): 29760
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

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