Anticorps Polyclonal de lapin anti-BLOC1S1-Specific

BLOC1S1-Specific Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 19687-1-AP

Synonymes

BLOC1S1, Biogenesis of lysosome-related organelles complex 1 subunit 1, BLOC 1 subunit 1, BLOC-1 subunit 1, BLOS1

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Galerie de données de validation

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  WB analysis of human brain using 19687-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19687-1-AP

  IP result of anti-BLOC1S1-Specific (IP:19687-1-AP, 3ug; Detection:19687-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human small intestine using 19687-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 19687-1-AP (BLOC1S1-Specific Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

19687-1-AP cible BLOC1S1-Specific dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: biogenesis of lysosomal organelles complex-1, subunit 1
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: NM_001487
• Symbole du gène: BLOC1S1
• Identification du gène (NCBI): 2647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BLOC1S1, also named as BLOS1, GCN5L1 and RT14, belongs to the BLOC1S1 family. BLOC1S1 may be involved in the biogenesis of specialized organelles of the endosomal-lysosomal system. The antibody is specific to NLOC1S1.

Publications

Species	Application	Title
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
human	WB	EMBO J; Snapin promotes HIV-1 transmission from dendritic cells by dampening TLR8 signaling.; Authors - Elham Khatamzas
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
human	WB	Oxid Med Cell Longev; General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease.; Authors - Tingting Lv
	WB,IP,IHC,IF	FEBS J; A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.; Authors - Daria Sicari

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