Anticorps Polyclonal de lapin anti-BMI1

• Phare
• Validé par KD/KO

BMI1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

34

N° de cat : 10832-1-AP

Synonymes

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

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Galerie de données de validation

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  WB analysis of HEK-293 using 10832-1-AP

  WB results of BMI antibody (10832-1-AP, 1:20000) with si-Control and si-BMI transfected HEK293 cells..

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  WB analysis of U-937 using 10832-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 10832-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10832-1-AP (BMI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 10832-1-AP

  IP Result of anti-BMI1 (IP:10832-1-AP, 4ug; Detection:10832-1-AP 1:500) with U-937 cells lysate 4000ug.

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10832-1-AP (BMI1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 10832-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL34 antibody 10832-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellule NCCIT, cellules HEK-293, cellules K-562, tissu cérébral de souris
• Résultats positifs en IP: cellules U-937
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

10832-1-AP cible BMI1 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMI1 Protéine recombinante Ag1286
• Nom complet: BMI1 polycomb ring finger oncogene
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-43 kDa
• Numéro d’acquisition GenBank: BC011652
• Symbole du gène: BMI1
• Identification du gène (NCBI): 648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Publications

Species	Application	Title
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran ; KD Validated
human	WB	Oncogene; Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.; Authors - Yang Chen
mouse	WB,CoIP,ChIP	J Neurosci; Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.; Authors - Kim Mee-Ohk MO
human	WB	Int J Cancer; SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.; Authors - S-L Xie
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei

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