Anticorps Monoclonal anti-BMP2

BMP2 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc et plus (2)

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

35

CloneNo.

1C5C2

N° de cat : 66383-1-Ig

Synonymes

BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

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Galerie de données de validation

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  WB analysis of pig bone marrow using 66383-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66383-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 66383-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de moelle osseuse de porc, cellules Caco-2, cellules HeLa
• Résultats positifs en IHC: tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

66383-1-Ig cible BMP2 dans les applications de WB, IHC, IF, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BMP2 Protéine recombinante Ag13501
• Nom complet: bone morphogenetic protein 2
• Masse moléculaire calculée: 396 aa, 44 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC069214
• Symbole du gène: BMP2
• Identification du gène (NCBI): 650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Publications

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions.; Authors - Haifang Li
human	WB	Angiogenesis; Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.; Authors - Vivien Becker
mouse	WB	Phytother Res; Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes; Authors - Jingwen Liu
human	WB	Oncogenesis; Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.; Authors - Lin Lu
rat	WB	J Hypertens; Saturated fatty acid promotes calcification via suppressing SIRT6 expression in vascular smooth muscle cells; Authors - Yafen Tao
human	WB	Front Oncol; Increased Methyl-CpG-Binding Domain Protein 2 Promotes Cigarette Smoke-Induced Pulmonary Hypertension.; Authors - Jixing Wu