Anticorps Polyclonal de lapin anti-BMP6

BMP6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 55421-1-AP

Synonymes

BMP-6, BMP 6, VG 1 related protein, VG 1 R, Bone Morphogenic Protein 6

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Galerie de données de validation

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  WB analysis using 55421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55421-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HL-60, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

55421-1-AP cible BMP6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bone morphogenetic protein 6
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: NM_001718
• Symbole du gène: BMP6
• Identification du gène (NCBI): 654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bone morphogenetic protein 6 (BMP6) is also named as VG-1-related protein (VG-1-R, VGR-1) and VGR, and belongs to the TGF-beta family (PMID:33878365). Growth factor of the TGF-beta superfamily plays essential roles in many developmental processes including cartilage and bone formation (PMID:31019025). BMP6 participates in iron homeostasis, inhibits invasion by increasing adhesions and cell-cell type interactions and induces angiogenesis directly on vascular endothelial cells. BMP6 is coded by a tumor suppressor gene whose subexpression is related to the development and cancer progression; during neoplastic processes, methylation is the main mechanism by which gene silencing occurs (PMID:34706618). BMP6 plays an important role in the regulation of hepcidin/HAMP expression and iron metabolism by acting as a ligand for hemojuvelin/HJV (PMID:26582087). BMP6 initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B (PMID:18070108).

Publications

Species	Application	Title
mouse	WB	J Neuroimmune Pharmacol; Ghrelin Induces Ferroptosis Resistance and M2 Polarization of Microglia to Alleviate Neuroinflammation and Cognitive Impairment in Alzheimer's Disease; Authors - Yaoxue Guo
human	WB	Cell Tissue Res; Bone morphogenetic protein 6 (BMP6) antagonises experimental proliferative vitreoretinopathy established by TGF-β2 stimulation in retinal pigment epithelial cells through modulation of the p38 and JNK MAPK pathways; Authors - Xuan Liu
human,rat	IHC	J Pharmacol Sci; Icariin alleviates diabetic renal interstitial fibrosis aggravation by inhibiting miR-320a-3p targeting BMP6; Authors - Kaiwei Wang
human	WB	J Assist Reprod Genet; BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis; Authors - Qihui Liang
mouse	WB	Geroscience; Tilorone attenuates high-fat diet-induced hepatic steatosis by enhancing BMP9-Smad1/5/8 signaling; Authors - Barnabas Horvath

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