Anticorps Polyclonal de lapin anti-BMPR1A

• Phare
• Validé par KD/KO

BMPR1A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 12702-1-AP

Synonymes

Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3, ALK3

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Galerie de données de validation

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  WB analysis using 12702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12702-1-AP

  WB result of BMPR1A antibody (12702-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMPR1A transfected MCF-7 cells.

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  WB analysis of human skeletal muscle using 12702-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12702-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12702-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12702-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12702-1-AP

  IP result of anti-BMPR1A (IP:12702-1-AP, 4ug; Detection:12702-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IF Staining of HeLa using 12702-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 12702-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules K-562, cellules MCF-7, cellules U2OS, tissu cardiaque humain, tissu de muscle squelettique humain, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HeLa, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

12702-1-AP cible BMPR1A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMPR1A Protéine recombinante Ag3410
• Nom complet: bone morphogenetic protein receptor, type IA
• Masse moléculaire calculée: 532 aa, 60 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC028383
• Symbole du gène: BMPR1A
• Identification du gène (NCBI): 657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo
human	IP	Sci Signal; Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells.; Authors - Sreeharsha Gurrapu
mouse	WB,IF	Front Cell Dev Biol; Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.; Authors - Chunlei Shao
mouse	WB	PLoS One; Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro.; Authors - Shasha Zhu
human	WB	Biochim Biophys Acta Gen Subj; Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels; Authors - Tianmiao Huang
rat	WB	Sci Rep; Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.; Authors - Yan Zeng

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