Anticorps Monoclonal anti-BORIS

BORIS Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2B12E2

N° de cat : 60079-1-Ig

Synonymes

CTCFL, 2B12E2, CCCTC-binding factor, CT27, CTCF paralog

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Galerie de données de validation

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  WB analysis of HeLa using 60079-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60079-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 60079-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60079-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SMMC-7721
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60079-1-Ig cible BORIS dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BORIS Protéine recombinante Ag1003
• Nom complet: CCCTC-binding factor (zinc finger protein)-like
• Masse moléculaire calculée: 82 kDa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC130486
• Symbole du gène: BORIS/CTCFL
• Identification du gène (NCBI): 140690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Publications

Species	Application	Title
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao

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