Anticorps Polyclonal de lapin anti-BPTF

BPTF Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 28016-1-AP

Synonymes

BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301

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Galerie de données de validation

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  IHC staining of human breast cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28016-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BPTF antibody (28016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

28016-1-AP cible BPTF dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BPTF Protéine recombinante Ag27761
• Nom complet: bromodomain PHD finger transcription factor
• Masse moléculaire calculée: 338 kDa
• Numéro d’acquisition GenBank: NM_004459
• Symbole du gène: BPTF
• Identification du gène (NCBI): 2186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)

Publications

Species	Application	Title
human	WB,IHC,ChIP	Biomolecules; Lumbrokinase Extracted from Earthworms Synergizes with Bevacizumab and Chemotherapeutics in Treating Non-Small Cell Lung Cancer by Targeted Inactivation of BPTF/VEGF and NF-κB/COX-2 Signaling; Authors - Chunyu Hua

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