Anticorps Polyclonal de lapin anti-BRD3

• Phare
• Validé par KD/KO

BRD3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 11859-1-AP

Synonymes

BRD3, bromodomain containing 3, KIAA0043, ORFX, RING3 like protein, RING3L

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Galerie de données de validation

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  WB analysis using 11859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11859-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11859-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11859-1-AP

  IP Result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11859-1-AP (BRD3 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules COLO 320, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 1 publications below

Informations sur le produit

11859-1-AP cible BRD3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRD3 Protéine recombinante Ag2433
• Nom complet: bromodomain containing 3
• Masse moléculaire calculée: 556 aa, 61 kDa
• Poids moléculaire observé: 95-105 kDa
• Numéro d’acquisition GenBank: BC032124
• Symbole du gène: BRD3
• Identification du gène (NCBI): 8019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).

Publications

Species	Application	Title
human	WB	Nat Med; Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.; Authors - Xiangpeng Dai
human	WB	Sci Adv; Light-induced control of protein destruction by opto-PROTAC.; Authors - Jing Liu
human	WB	J Am Chem Soc; Cancer Selective Target Degradation by Folate-Caged PROTACs.; Authors - Jing Liu
human	WB	Nat Commun; Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer.; Authors - Wenyu Wang
human	WB	Nat Commun; A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.; Authors - Satoshi Yamanaka
human	WB	Nat Commun; The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.; Authors - Zhicheng Shao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zehua (Verified Customer) (02-06-2019)

Works well on fractionation lysates of MCF-7 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

BRD3 Antibody Western Blot, validation (1:1000 dilution) in MCF-7 (Cat no:11859-1-AP)