Anticorps Polyclonal de lapin anti-BRD8

• Phare
• Validé par KD/KO

BRD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 10476-1-AP

Synonymes

TrCP120, SMAP2, SMAP, p120, Bromodomain-containing protein 8

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Galerie de données de validation

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  WB analysis of HeLa using 10476-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10476-1-AP

  WB result of BRD8 antibody (10476-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRD8 transfected HeLa cells.

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  WB analysis using 10476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10476-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10476-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10476-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10476-1-AP (BRD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10476-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (10476-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HuH-7, tissu cérébral humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10476-1-AP cible BRD8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRD8 Protéine recombinante Ag0790
• Nom complet: bromodomain containing 8
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120-170 kDa
• Numéro d’acquisition GenBank: BC008039
• Symbole du gène: BRD8
• Identification du gène (NCBI): 10902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)

Publications

Species	Application	Title
human	WB	Nature; ANP32E is a histone chaperone that removes H2A.Z from chromatin.; Authors - Arnaud Obri
human	WB	Arch Biochem Biophys; BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5.; Authors - Zhaoxiang Yu

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