Anticorps Polyclonal de lapin anti-BRD8

BRD8 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IF,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10476-1-AP

Synonymes

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

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Galerie de données de validation

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  WB analysis of HeLa using 10476-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10476-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10476-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10476-1-AP

  IP Result of anti-BRD8 (IP:10476-1-AP, 4ug; Detection:10476-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 10476-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10476-1-AP (BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10476-1-AP cible BRD8 dans les applications de WB, IP, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRD8 Protéine recombinante Ag0790
• Nom complet: bromodomain containing 8
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC008039
• Symbole du gène: BRD8
• Identification du gène (NCBI): 10902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

Publications

Species	Application	Title
human	WB	Nature; ANP32E is a histone chaperone that removes H2A.Z from chromatin.; Authors - Arnaud Obri
human	WB	Arch Biochem Biophys; BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5.; Authors - Zhaoxiang Yu