Anticorps Polyclonal de lapin anti-MCPH/BRIT1

• Phare
• Validé par KD/KO

MCPH/BRIT1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 11962-1-AP

Synonymes

BRIT1, MCPH1, MCT, Microcephalin, microcephalin 1

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Galerie de données de validation

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  WB analysis of HeLa using 11962-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11962-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11962-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11962-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11962-1-AP (BRIT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11962-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11962-1-AP (BRIT1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules MDA-MB-453s, tissu ovarien de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

11962-1-AP cible MCPH/BRIT1 dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCPH/BRIT1 Protéine recombinante Ag2567
• Nom complet: microcephalin 1
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 66 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC030702
• Symbole du gène: MCPH1
• Identification du gène (NCBI): 79648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )

Publications

Species	Application	Title
human	WB	FASEB J; Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.; Authors - Stephanie K Meyer ; KD Validated
human	WB, IHC	Stem Cell Res; Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.; Authors - Zerui Wang ; KO Validated
human	WB	FEBS J; Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.; Authors - Maria Arroyo ; KD Validated
human	WB	Genes (Basel); MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.; Authors - María Arroyo ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (11-10-2022)

I compared several commercial antibodies for MCPH1 and this was the only one that was able to detect both isoforms in Western blotting. Importantly, these bands disappeared after treatment of cells with MCPH1 siRNA.

• Applications: Western Blot
• Cell Tissue Type: Human cell lines