Anticorps Polyclonal de lapin anti-BCL2L1

BCL2L1 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Avis (1)

N° de cat : CL647-26967

Synonymes

Bcl-xL, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1, Bcl-2-like protein 1

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Galerie de données de validation

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  IF Staining of HepG2 using CL647-26967

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 Bcl-XL antibody (CL647-26967) at dilution of 1:200.

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  FC experiment of Jurkat using CL647-26967

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Bcl-XL (CL647-26967) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-26967 cible BCL2L1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCL2L1 Protéine recombinante Ag25604
• Nom complet: BCL2-like 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC019307
• Symbole du gène: BCL2L1
• Identification du gène (NCBI): 598
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexia (Verified Customer) (07-25-2023)

Murine thymocytes were stained for 90 minutes at 4°C in the dark. The stain is extremely convincing compared to FMO.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Thymocytes

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