Anticorps Monoclonal anti-Bcl2

• Phare
• Validé par KD/KO

Bcl2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(266)

CloneNo.

1B3F7

Avis (1)

N° de cat : 68103-1-Ig

Synonymes

Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2

---

Galerie de données de validation

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68103-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 68103-1-Ig

  C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68103-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 68103-1-Ig

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 68103-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 68103-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 68103-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 68103-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 68103-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 68103-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules C2C12, cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de rat, tissu cérébral de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu cutané de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 253 publications below
• IHC: See 12 publications below
• IF: See 7 publications below

Informations sur le produit

68103-1-Ig cible Bcl2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Chèvre, porc, poulet, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24261
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Publications

Species	Application	Title
	WB	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB	Bioact Mater; Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma; Authors - Yuhan Wang
mouse	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
mouse	WB	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
	WB	Cancer Res; PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT; Authors - Wenchao Zhang
mouse	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Amandine (Verified Customer) (02-07-2024)

20ug of protein denatured or not, primary antibody 1/5000 RT and secondary antibody 1/5000 2h RT. Chemiluminescence revelation with 10min of exposition

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: ImCC

Bcl2 Antibody Western Blot validation (1/5000 dilution) in ImCC (Cat no:68103-1-Ig)

{{ptg:RelatedPrimaryAntibodies}}