Anticorps Recombinant de lapin anti-Beta Catenin

Beta Catenin Recombinant Antibody for WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, porc, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2J24

N° de cat : 80488-1-RR

Synonymes

2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1

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Galerie de données de validation

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  WB analysis using 80488-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80488-1-RR

  zebrafish tissue was subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80488-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 80488-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  ChIP experiment of HCT 116 using 80488-1-RR

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of β-Catenin (80488-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, tissu cérébral de porc, tissu de poisson-zèbre
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en ChIP-qPCR: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

80488-1-RR cible Beta Catenin dans les applications de WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, poisson-zèbre, porc, rat, souris

• Réactivité: Humain, poisson-zèbre, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC058926
• Symbole du gène: Beta Catenin
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
human	WB,IF,CoIP	Cell Biol Toxicol; M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway; Authors - Chao Zhang
human	WB	Discov Oncol; miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1; Authors - Jing Li

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