Anticorps Monoclonal anti-Beta Tubulin

Beta Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, namatode, poisson-zèbre, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1D4A4

N° de cat : 66240-1-PBS

Synonymes

Tubulin beta 3 chain, Tubulin beta III, 1D4A4, TUBB4, Tubulin beta-3 chain

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Galerie de données de validation

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  WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  IP result of anti-Beta Tubulin (IP:66240-1-Ig, 5ug; Detection:66240-1-Ig 1:1000) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66240-1-Ig (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66240-1-PBS cible Beta Tubulin dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, namatode, poisson-zèbre, porc, rat, souris

• Réactivité: Humain, namatode, poisson-zèbre, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Tubulin Protéine recombinante Ag0117
• Nom complet: tubulin, beta 3
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000748
• Symbole du gène: TUBB3
• Identification du gène (NCBI): 10381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The alpha and beta tubulins (+/- 55 kDa MW) are homologous but are not identical. Beta tubulins have been widely used as loading control.

What is the molecular weight of beta-tubulin? Are there any isoforms of beta-tubulin?

The molecular weight of tubulin is 50-52 kDa. Humans have eight beta-tubulin isotypes, encoded by different genes, that differ in their C-terminal sequences. They have different tissue expression profiles and can rise to microtubules of different properties (PMID: 20191564).

How to use beta-tubulin as a loading control

Beta-tubulin is one of the most commonly used references as a loading control for cell lysates in western blotting. It is abundantly expressed across various tissues and developmental stages and highly conserved across species. However, since some variability has been observed in the expression levels of commonly used housekeeping genes (PMID: 15627964), it is recommended that more than one loading control antibody is used while developing new assays. More information can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

What drugs can influence beta-tubulin and organization of microtubules?

Many drugs that affect microtubule dynamics target beta-tubulin, mainly by interfering with the GTP hydrolysis (PMID: 21381049). Paclitaxel (Taxol) is used to stabilize microtubules by slowing down their depolymerization, while colchicine and vinca alkaloids (vinblastine) destabilize microtubules. They are used in research and also in the clinic as anti-cancer agents.

Is beta-tubulin post-translationally modified?

Yes, tubulins are subject to extensive post-translational modifications (PTMs) that affect the organization of microtubules and their dynamics. The most common modifications include polyglutamylation, polyglycylation, polyamination, glycososylation, glycation, phosphorylation, and acetylation (PMID: 24801181 and 25468068).

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