Anticorps Recombinant de lapin anti-Beta galactosidase

• Phare
• Validé par KD/KO

Beta galactosidase Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240788F1

N° de cat : 83706-6-RR

Synonymes

GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP

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Galerie de données de validation

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  WB analysis of A549 using 83706-6-RR

  WB result of Beta galactosidase antibody (83706-6-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta galactosidase transfected A549 cells.

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  WB analysis using 83706-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83706-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83706-6-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83706-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HepG2, cellules K-562, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83706-6-RR cible Beta galactosidase dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Beta galactosidase Protéine recombinante Ag7792
• Nom complet: galactosidase, beta 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 62-67 kDa
• Numéro d’acquisition GenBank: BC007493
• Symbole du gène: GLB1/Beta-galactosidase
• Identification du gène (NCBI): 2720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.

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