Anticorps Polyclonal de lapin anti-EDRF1

EDRF1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 21883-1-AP

Synonymes

C10orf137, EDRF1, RP11 383C5.6

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Galerie de données de validation

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  WB analysis using 21883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21883-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21883-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21883-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21883-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, cellules PC-3, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21883-1-AP cible EDRF1 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDRF1 Protéine recombinante Ag16441
• Nom complet: chromosome 10 open reading frame 137
• Masse moléculaire calculée: 1238 aa, 139 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC107479
• Symbole du gène: C10orf137
• Identification du gène (NCBI): 26098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.