Anticorps Polyclonal de lapin anti-C14orf166

• Phare
• Validé par KD/KO

C14orf166 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 19848-1-AP

Synonymes

C14orf166, CGI 99, CGI99, CLE, CLE7, LCRP369, RLLM1, RTRAF, UPF0568 protein C14orf166

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Galerie de données de validation

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  WB analysis using 19848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19848-1-AP

  WB result of C14orf166 antibody (19848-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C14orf166 transfected A549 cells.

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  WB analysis of mouse pancreas using 19848-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19848-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19848-1-AP

  IP Result of anti-C14orf166 (IP:19848-1-AP, 3ug; Detection:19848-1-AP 1:700) with Jurkat cells lysate 1080ug.

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  IHC staining of human breast cancer using 19848-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19848-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 19848-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19848-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 19848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using C14orf166 antibody (19848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, tissu pancréatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below

Informations sur le produit

19848-1-AP cible C14orf166 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C14orf166 Protéine recombinante Ag13900
• Nom complet: chromosome 14 open reading frame 166
• Masse moléculaire calculée: 244 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC001722
• Symbole du gène: C14orf166
• Identification du gène (NCBI): 51637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing.; Authors - Teruhiko Suzuki
human	WB,IHC	Biosci Rep; Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small cell lung cancer.; Authors - Yan-Wu Zhou
human	WB,IHC	J Transl Med; C14orf166 overexpression correlates with tumor progression and poor prognosis of breast cancer.; Authors - Tuck-Yun Cheang ; KD Validated
human	WB, IHC	Tumour Biol; C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer.; Authors - Weijing Zhang
human	WB, IHC	Tumour Biol; Overexpression of chromosome 14 open reading frame 166 correlates with disease progression and poorer prognosis in human NPC.; Authors - Lin Yang
human	WB	Mol Med Rep; Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma.; Authors - Yan-Wu Zhou