Anticorps Polyclonal de lapin anti-C19orf21

• Phare
• Validé par KD/KO

C19orf21 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 26338-1-AP

Synonymes

MISP, Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein

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Galerie de données de validation

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  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26338-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HaCaT using 26338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using C19orf21 antibody (26338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2
• Résultats positifs en IF/ICC: cellules HaCaT,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

26338-1-AP cible C19orf21 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C19orf21 Protéine recombinante Ag23712
• Nom complet: chromosome 19 open reading frame 21
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC052236
• Symbole du gène: C19orf21
• Identification du gène (NCBI): 126353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.

Publications

Species	Application	Title
human	WB	Front Oncol; Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma.; Authors - Xinyang Huang
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
mouse	WB,IF	J Vet Med Sci; Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice; Authors - Koki Hiura ; KO Validated
human	WB,IP,IF	Int J Mol Sci; Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway; Authors - Koki Hiura ; KD Validated
human	WB	Front Pharmacol; Protein and metabolic profiles of tyrosine kinase inhibitors co-resistant liver cancer cells; Authors - Zengbin Wang
human	WB	Adv Sci (Weinh); MISP Suppresses Ferroptosis via MST1/2 Kinases to Facilitate YAP Activation in Non-Small Cell Lung Cancer; Authors - Fuquan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, HaCaT, U2OS

C19orf21 Antibody Western Blot, validation (1:1000 dilution) in 293T, HaCaT, U2OS (Cat no:26338-1-AP)

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