Anticorps Polyclonal de lapin anti-C19orf52

• Phare
• Validé par KD/KO

C19orf52 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 25652-1-AP

Synonymes

Mitochondrial import inner membrane translocase subunit Tim29, TIM29, TIMM29

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 25652-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25652-1-AP (C19orf52 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 25652-1-AP

  IP result of anti-C19orf52 (IP:25652-1-AP, 3ug; Detection:25652-1-AP 1:700) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 25652-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25652-1-AP (C19orf52 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25652-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25652-1-AP (C19orf52 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below

Informations sur le produit

25652-1-AP cible C19orf52 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C19orf52 Protéine recombinante Ag22251
• Nom complet: chromosome 19 open reading frame 52
• Masse moléculaire calculée: 260 aa, 29 kDa
• Poids moléculaire observé: 29-32 kDa
• Numéro d’acquisition GenBank: BC005075
• Symbole du gène: C19orf52
• Identification du gène (NCBI): 90580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.; Authors - Zhilin Hu
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
human	WB	J Med Virol; TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression; Authors - Lin Guo ; KD Validated
human	WB	J Mol Biol; Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex.; Authors - Arianna Barchiesi
human	WB	Mol Biol Cell; Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.; Authors - Katarzyna Justyna Chojnacka
human	WB	FEBS Lett; TIM29 is a subunit of the human carrier translocase required for protein transport.; Authors - Sylvie Callegari ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wenhui (Verified Customer) (01-02-2025)

This antibody has been working great with WB. We can detect clear bands with this antibody.I'd recommended it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiac tissues and cardiomyocytes

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