Anticorps Polyclonal de lapin anti-C1QA

C1QA Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11602-1-AP

Synonymes

C1qA

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Galerie de données de validation

• 

  WB analysis of human plasma using 11602-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse serum using 11602-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11602-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11602-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 11602-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11602-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11602-1-AP

  IP Result of anti-C1QA (IP:11602-1-AP, 4ug; Detection:11602-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: plasma humain, sérum de souris, tissu cardiaque humain, tissu de côlon humain, tissu hépatique de souris, tissu hépatique humain, tissu pulmonaire humain, tissu splénique humain
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

11602-1-AP cible C1QA dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1QA Protéine recombinante Ag2141
• Nom complet: complement component 1, q subcomponent, A chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC030153
• Symbole du gène: C1QA
• Identification du gène (NCBI): 712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in mouse models of Parkinson's disease; Authors - Wenlong Zhang
mouse	WB	J Neurosci; Immune quiescence of the brain is set by astroglial connexin 43.; Authors - Anne-Cécile Boulay
rat	IF,WB	Neurochem Res; Blocking Two-Pore Domain Potassium Channel TREK-1 Inhibits the Activation of A1-Like Reactive Astrocyte Through the NF-κB Signaling Pathway in a Rat Model of Major Depressive Disorder; Authors - Ting Cong
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	Oncol Lett; Role of uromodulin and complement activation in the progression of kidney disease.; Authors - Fei Shen

Produits associés

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66268-1-Ig

C1qC Polyclonal antibody

16889-1-AP

C1R Polyclonal antibody

17346-1-AP

C1S Polyclonal antibody

14554-1-AP