Anticorps Polyclonal de lapin anti-C1QA

C1QA Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 11602-1-AP

Synonymes

Complement C1q A chain

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Galerie de données de validation

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  WB analysis using 11602-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 11602-1-AP (C1qA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 11602-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11602-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11602-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11602-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 11602-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 11602-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (11602-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: plasma humain, tissu cardiaque humain, tissu de côlon humain, tissu hépatique humain, tissu pulmonaire humain, tissu splénique humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 5 publications below

Informations sur le produit

11602-1-AP cible C1QA dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1QA Protéine recombinante Ag2141
• Nom complet: complement component 1, q subcomponent, A chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC030153
• Symbole du gène: C1QA
• Identification du gène (NCBI): 712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
	WB	Brain Behav Immun; PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice; Authors - Qian Zhao
	WB	Neuron; TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4; Authors - Maud Gratuze
	WB	Cell Mol Life Sci; Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in mouse models of Parkinson's disease; Authors - Wenlong Zhang
	WB	J Neurosci; Immune quiescence of the brain is set by astroglial connexin 43.; Authors - Anne-Cécile Boulay
	WB	Life Sci; Cognitive impairment in Alzheimer's disease FAD4T mouse model: Synaptic loss facilitated by activated microglia via C1qA; Authors - Cui Zhang
human	WB,IF	CNS Neurosci Ther; STING Driving Synaptic Phagocytosis of Hippocampal Microglia/Macrophages Contributes to Cognitive Impairment in Sepsis-Associated Encephalopathy in Mice; Authors - Xin Lv

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