Anticorps Polyclonal de lapin anti-C1QBP

• Phare
• Validé par KD/KO

C1QBP Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 24474-1-AP

Synonymes

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32

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Galerie de données de validation

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  WB analysis using 24474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24474-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24474-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24474-1-AP (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24474-1-AP (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

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  IF Staining of NIH/3T3 using 24474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using C1QBP antibody (24474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 24474-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human C1QBP (24474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules C2C12, cellules HCT 116, cellules HeLa, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

24474-1-AP cible C1QBP dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1QBP Protéine recombinante Ag19773
• Nom complet: complement component 1, q subcomponent binding protein
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC013731
• Symbole du gène: C1QBP
• Identification du gène (NCBI): 708
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

Publications

Species	Application	Title
human	WB,IF	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang ; KO Validated
human	WB	Proc Natl Acad Sci U S A; A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress; Authors - Shuang Feng
human	WB	J Control Release; PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.; Authors - Jiang Ni
mouse	WB	Cancer Sci; Complement C1q binding protein regulates T cells' mitochondrial fitness to affect their survival, proliferation, and anti-tumor immune function.; Authors - Hui Tian ; KO Validated
	WB,IF	Int J Mol Sci; The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.; Authors - Jianyu Ma ; KD Validated
rat	WB	Biol Pharm Bull; Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.; Authors - Yue-Hua Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h.Primary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293t

C1QBP Antibody Western Blot, validation (1:500 dilution) in HEK293t (Cat no:24474-1-AP)

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