Anticorps Monoclonal anti-C1qC

C1qC Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1C5B4

N° de cat : 66268-1-Ig

Synonymes

Complement C1q C chain, C1Q C, 1C5B4, C1QG

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Galerie de données de validation

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  WB analysis of human plasma using 66268-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66268-1-Ig (C1QC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 66268-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using C1qC antibody (66268-1-Ig, Clone: 1C5B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66268-1-Ig cible C1qC dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: C1qC Protéine recombinante Ag10233
• Nom complet: complement component 1, q subcomponent, C chain
• Masse moléculaire calculée: 245 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC009016
• Symbole du gène: C1QC
• Identification du gène (NCBI): 714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
rat	WB	J Ethnopharmacol; Network pharmacology and experimental validation-based approach to understand the effect and mechanism of Taohong Siwu Decoction against ischemic stroke.; Authors - Lingyu Pan
human	WB	Onco Targets Ther; Prognostic Implications of the Complement Protein C1Q and Its Correlation with Immune Infiltrates in Osteosarcoma.; Authors - Hanji Huang
human	IF	PLoS Genet; Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer; Authors - Tiantian Ji
human	IF	Cell Rep Med; Phenotypic plasticity and increased infiltration of peripheral blood-derived TREM1+ mono-macrophages following radiotherapy in rectal cancer; Authors - Haihong Wang

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