Anticorps Polyclonal de lapin anti-C1S

C1S Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 14554-1-AP

Synonymes

C1 esterase, C1S, Complement C1s subcomponent

---

Galerie de données de validation

• 

  WB analysis of human plasma using 14554-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14554-1-AP

  rat plasma lysates were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 14554-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: rat plasma, plasma humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14554-1-AP cible C1S dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: C1S Protéine recombinante Ag5977
• Nom complet: complement component 1, s subcomponent
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC056903
• Symbole du gène: C1S
• Identification du gène (NCBI): 716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

Publications

Species	Application	Title
mouse	IF	Int J Mol Sci; Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina; Authors - Anne Enzbrenner
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig
mouse	WB,IF	Cell Rep; Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.; Authors - Diana Pauly
human	IHC,IF	J Transl Med; Comprehensive investigation of malignant epithelial cell-related genes in clear cell renal cell carcinoma: development of a prognostic signature and exploration of tumor microenvironment interactions; Authors - Songyang Liu
	IF	Cancer Cell Int; Association between cancer-associated fibroblasts and prognosis of neoadjuvant chemoradiotherapy in esophageal squamous cell carcinoma: a bioinformatics analysis based on single-cell RNA sequencing; Authors - Zhao Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Asimenia (Verified Customer) (11-14-2024)

The signal of this staining was clear and with minimal background on the cells or the surrounding tissue.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hiPSC-derived microglia

C1S Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in hiPSC-derived microglia (Cat no:14554-1-AP)

{{ptg:RelatedPrimaryAntibodies}}