Anticorps Polyclonal de lapin anti-METTL18

METTL18 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 25553-1-AP

Synonymes

C1orf156, Arsenic-transactivated protein 2, AsTP2, EC:2.1.1.85, Histidine protein methyltransferase 1 homolog

---

Galerie de données de validation

• 

  WB analysis of A431 using 25553-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25553-1-AP (C1orf156 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25553-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25553-1-AP (C1orf156 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 25553-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL18 antibody (25553-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules PC-3
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

25553-1-AP cible METTL18 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL18 Protéine recombinante Ag22148
• Nom complet: chromosome 1 open reading frame 156
• Masse moléculaire calculée: 372 aa, 42 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC008679
• Symbole du gène: METTL18
• Identification du gène (NCBI): 92342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL18 belongs to a family of protein lysine methyltransferases that methylate nonhistone proteins. Methyltransferases catalyze the transfer of a methyl group from a donor, generally S-adenosyl-L-methionine, to acceptor molecules, including proteins, DNA, RNA, lipids, and small metabolites. METTL18 is involved in peptidyl-lysine monomethylation.

Publications

Species	Application	Title
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
human	WB	Front Oncol; Identification METTL18 as a Potential Prognosis Biomarker and Associated With Immune Infiltrates in Hepatocellular Carcinoma.; Authors - Tian-Hao Li

{{ptg:RelatedPrimaryAntibodies}}